1.
Homologous recombination-based DNA modification for large constructs
Trade
your original DNA for something new. This
innovative technology enables the following modifications for medium to large
sized vector constructs:
·
Point
mutations
·
DNA
Insertions
·
DNA
Deletions
·
DNA
Exchanges
Now
you can easily create any number of changes to your clone. For example, you can
make insertions of several kilobases to your clone and study how extra DNA
modifies the pathway or the disease phenotype. Alternatively, you can delete
fragments of DNA for transgenic analysis and discover the effects of loss of
function in cells. If proteins fascinate you, we can create mutations at
several points in the insert enabling you to monitor how varying protein
structures play out in the cell.
Additionally, if you want to add expression reporters, protein tags in
order to monitor dynamic changes in real time inside the cell, we can easily do
that. All these can variously be performed to modify BAC, PAC clones
eliminating the use of special libraries.
The techniques we employ provide an easy avenue to comprehensively analyze gene
function in vivo.
Since homologous recombination mediated DNA modification is restriction
site independent, DNA ligation is unnecessary. Even when no compatible
restriction sites are available during cloning or sequence assembly our
technique allows you to modify target DNA. With conventional PCR based
site-directed- mutagenesis the whole vector construct including the target
sequence has to be amplified by PCR
which is not technically feasible. With our expertise you can avoid unwanted
mutations while making base alterations because the fidelity of sequences other
than the mutation or insertion site is not disrupted.
2. Retrofit old BAC/PAC clones and make them eukaryote-system-ready!
Until
now, BAC clones have been used predominantly in genome sequence and structure
analysis. However, these first generation BAC/PAC clones are not conducive for
functional genomic analysis in eukaryotic model organisms - lacking the
characteristic eukaryotic elements that facilitate transfection, episomal
stability and counter selection.
We are pleased to announce a homologous recombination-based gene fragment
exchange system to modify old BAC clones with the necessary traits for
efficient manipulation in eukaryotes. With this service we now offer a
variety of options to engineer your BAC/PAC clone including, but not limited
to, the right antibiotic selection marker (e.g., hygromycin) in eukaryotes,
customer provided luminescent reporter gene [GFP or luciferase] for
photodynamic imaging, identification and sorting of transfected cells. All this
with an origin of replication tailored to work in eukaryotic systems. Finally,
you can make your BAC clone ready for transgenic research, translocation
studies in oncology, comparative genomic hybridization (CGH), Fluorescent in
situ hybridization (FISH) analysis in diagnostics and so much more!
All this and much more at Bio S&T.
Where we do it all for you!
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