Meet Bio S&T A foreword by our CEO Upcoming conferences BAC library expertise 3 options BAC sequencing RFQ cDNA and DNA libraries Lambda cDNA/genomic Plasmid cDNA/genomic Normalized cDNA RFQ   Gene synthesis Deliverables and guarantees RFQ Mutagenesis Deliverables and guarantees RFQ DNA sequencing Individual sequencing PCR products END EST clones Whole genome Technology portfolio Drug screening APA technology Sequencing technology APAgene GOLD Walking Kits Retroviral products Other custom services Contact info Site map Our price: 1,499 / unit   One unit is equal to 10Kb (insert DNA + vector). The final cost will increase or decrease according to the final assembled genome size. The price does not include genome annotation.   Outline of the procedure: Cell culture of BAC clones. Extraction & Purification of BAC DNA. Determination of BAC Size (vector plus insertion).  Construction of Random Shotgun Library. Sequencing: - Template DNA Extraction & Purification. - Shotgun sequencing by universal primer. - Real time annotation reports: Assemble Contig Alignment Bridge (Linking) Clone Identification - Gap Closing: Determination of bridge clone sequence or, random amplified PCR products based on contig end specific primers.  Average genome coverage > 99%. Special offer: Clients who have constructed and screened a BAC library with Bio S&T, or will do so at a future date, will benefit from a preferred rate! Please contact us for more details!