Meet Bio S&T A foreword by our CEO Upcoming conferences BAC library expertise 3 options BAC sequencing RFQ cDNA and DNA libraries Lambda cDNA/genomic Plasmid cDNA/genomic Normalized cDNA RFQ Gene synthesis Deliverables and guarantees RFQ Mutagenesis Deliverables and guarantees RFQ DNA sequencing Individual sequencing PCR products END EST clones Whole genome Technology portfolio Drug screening APA technology Sequencing technology APAgene GOLD Walking Kits Retroviral products Other custom services Contact info Site map    Lambda cDNA/DNA libraries (For plasmid cDNA/DNA libraries click here!) Lambda cDNA library For a limited time, order a standard  lambda cDNA library for the low price of $3,459 (for 1,000,000 clones). Pricing is available for a higher amount of clones, please contact us for more information! Normalized cDNA libraries are available! NEW feature: Duo-range libraries! Receive 2 sub-libraries of 750,000 clones each. Each will have a differrent insert size range of your choice (ex: 500bp-2Kb and 2Kb-4Kb). This maximizes flexibility and representability. Add this feature for an additional $499. Service highlights: cDNA libraries are produced using our proprietary technology, which features full-length enriched, high quality size-fractionated, and directionally cloned cDNA. We guarantee the minimum requested 1,000,000 clones. 1st strand cDNA synthesis: 5’ adaptor primers will be added to full-length cDNAs during this step; an oligo-dT or a random primer based on the need of customer will be used. 2nd strand cDNA synthesis by primer extension.  Double stranded cDNAs will be amplified using low cycling PCR if necessary (optional). Size fractionation. Ligation to phage vector (customer supplied vector could be accepted but a fee may apply for modifications). Packaging. Quality control (average insert size and non-insert clone determination, number and percentage of recombinants). Customer will receive non-amplified cDNA stocks. Construction of a Genomic DNA Lambda library - Bio S&T Service Highlights: Price is $3,459 for 1 million clones. Bio S&T can clone large inserts ranging in size from 9-23 kb into a lamdba vector.  The Lambda DASH II Vector (Stratagene) will be used for genomic lambda library construction. Instructions will be followed as specified by manufacturer (Stratagene). We do not guarantee average insert size but will attempt to obtain desired range of average insert size. Insert size can vary from about 9-23Kb, as specified in manufactures’ kit. We guarantee the minimum requested 1,000,000 clones. Genomic library preparation highlights: DNA purification (>100kb in average). DNA random shearing by enzymatic methods. Size fractionation. Sticky-end ligation to the vector.  Packaging and stock preparation. Customer will receive non-amplified phage stocks. Recombinant clones will be a minimum of 1 million.