BAC library construction

We guarantee a quick turnaround-time, unbeatable pricing and large inserts! 100% of our clients have found their target genes!

We offer (Please CLICK BELOW LINKS for more details!) :

1.Arrayed BAC library

2. Non-Arrayed BAC library

3.Screened BAC library

4. Pools

5. Filters

Our strengths:

  • We guarantee coverage.

  • We perform HMW DNA extraction at our facilities.

  • We have experience with plant, fungal, bacterial and animal libraries.

  • We have extensive experience with GC-rich organisms and marine bacteria.

  • Our arrayed BAC libraries are shipped in bar-coded microplates.

  • We have extensive experience shipping worldwide.

  • Shipment temperature of non-arrayed libraries is always monitored using a BLUE FLAG Temperature Data logger.

  • We provide technical support.

  • BAC libraries are the client's property and are never used internally, sold or distributed.

 A few libraries constructed in the past include: 

Starting material Insert size
Mouse tissue 180 Kb
Soy bean 150 Kb
Zeas Mays 150 Kb
Beetle 162 Kb
Human liver 215 Kb
P. brassicacearum 160 Kb
P. omnivora 185 Kb

Here is an exmaple of an insect BAC library (Mamestra configurata) with an average insert size of 178Kb.


Here is an exmaple of a fungal BAC library (P. omnivora) with an average insert size of 185Kb.

 

References are always available. Please CLICK HERE for more details!

 

New in 2008

On December 19th 2008, Bio S&T will draw a name from all our 2008 BAC library clients and winner will be entitled to a $1,000 US credit to be used in 2009 towards any of our services. All BAC library cients will be automatically entered and winner will be contacted on January 5th 2009.

 

Bio S&T will donate 2% of proceeds from every BAC library sold in 2008 to benefit The Montreal Children's Hospital.

Importance of a BAC library

BAC libraries are essential elements in doing large-scale genome research such as genome sequencing, physical maps, and gene cloning. Bacterial artificial chromosomes (BAC) cloning technology was developed in early 1990s and quickly gained popularity. This technology was used in applications for genomic analysis that included large-scale physical mapping and genomic sequencing although this technology was developed much later than Yeast Artificial Chromosome (YAC) and cosmid cloning technologies. This should be attributed to the technical advantages of BAC cloning over YAC and cosmid cloning system summarized as follows:

  • Bigger insert size (up to 300 kb) than cosmid and other plasmid system.

  • Much more stable than yeast.

  • Bacterial clones and libraries grow much faster than YAC.

  • Easier in handling and gridding, DNA preparation.

  • Allows for more efficient screening by either hybridization or PCR based screening.

The major applications of BAC library construction are in large-scale physical mapping and genomic sequencing and encompass the following applications:

Isolation of Gene clusters for entire metabolic pathway.

A BAC library can be employed to scan for gene pathways in drug discovery, gene pathways in various diseases, plant genetics and microbial metabolic pathways. A variety of applications derive from construction of BAC libraries including BAC Macro arrays, gene isolation by pooled PCR, homology based hybridization using various DNA probes.

Prepare FISH probes 

Fluorescence In Situ Hybridization (FISH) employs BAC vectors as probes to illumine and analyze tissue samples to understand chromosomal rearrangements, amplifications, deletions and aneusomies. Usually fluorescent probes for specific section(s) of a chromosome are used to label a locus of a chromosome within a cell sample where the specified genetic sequences are found. 

Complementation for map-based gene cloning using Binary vector in plants.

A BAC library can be used to interrogate the genetic structure of plant genomes as well as mutant genotype analysis using complementation techniques. This endows the scientist with a reliable tool to study global gene expression in plant species and to isolate individual genes.

Genomic walking

This allows direct genome sequencing using BAC libraries instead of sequencing directly from genomes, which is a very difficult proposition and allows study of gene structure using the entire genome.

Please contact us for more details!