ULTRAmix 2X PCR pre-mix
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ULTRAmix 2X PCR premix is an economical, convenient, all-in-one ready-to-use PCR premix (just add template + primers!), that can amplify templates of up to 5 kb.
We offer two convenient formats to allow clients worldwide to choose their preferred shipping method:
- $85 for 300 PCR reactions (#P500-300 product requires dry ice shipping; recommended for Canada and USA clients)
- $115 for 300 PCR reactions (#P500-301 product requires room temperature shipping; recommended for International clients)
ULTRAmix 2X PCR premix yields high-quality and consistent results in routine PCR reactions, as well as high-throughput PCR genotyping, colony PCR, RT-PCR and PCR cloning.
Figure 1: Amplification using ULTRAmix 2X PCR pre-mix
PCR reactions can be directly loaded onto an agarose gel without the additional need of loading buffer and dyes. Three dyes help visualize the electrophoresis progress:
- Blue dye (~4Kb)
- Red dye (~1Kb)
- Yellow dye (~0.1Kb)
As an added feature, a positive PCR Control Mix is included. When used with the ULTRAmix 2X PCR premix, a 3kb fragment will be amplified and serve as a positive control.
Components
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Components
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Catalogue
No.
|
50µl PCR
reactions
|
|
ULTRAmix 2X
PCR premix
PCR Control Mix
|
- PCR buffer with Mg2+
- dNTPs
- Proprietary PCR enhancer/stabilizer
- Gel loading dye
- High-quality Taq polymerase
- A mix containing forward primer, reverse primer and template DNA
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P500-300
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300
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ULTRAmix 2X PCR premix can be stored at -20oC for 12 months. It remains stable for up to ten (10) freeze-thaw cycles. However, we
strongly recommend that freeze-thaw cycles be minimized.
Quality control
PCR activity of ULTRAmix 2X PCR premix is tested to ensure consistent high performance and efficient routine PCR amplifications using a variety
of templates and primers. Nicking activity, endonuclease and exonuclease activity are not detected.
PCR Setup protocol for #P500-300
Click here to download our protocol for #P500-300. Protocol for #P500-301will be available April 12th 2010.
Call us (1-888-368-8368) or email us info@biost.com to order!
