APAgene™ GOLD walking kits

---   APAgene™ GOLD-RT Genome Walking Kits can now be shipped at room temperature !
---   International customers can save on shipping fees!

• Our kit was recently featured in Molecular Biotechnology
  (D. Cullen et al., 11 January 2011 pp. 1-13) where it rated
  the best amongst our competitors.

 

APAgene™ GOLD Genome Walking Kits are designed to rapidly (1 day!) and reliably amplify unknown genomic DNA using our patented APA technology. Also available, and on promotion, is our APAgene WALKING service.

 

APAgene™ GOLD is the ideal genomic tool because it allows you to carry out an entire range of molecular genetic experiments:

1. Isolate unknown genomic sequences flanking transgenes (including T-DNAs, gene traps, transposons), sequence-tagged sites (STSs) or expressed sequence tags (ESTs).
2. Isolate localized sequences flanking known sequences from large clones and genomic DNAs.
3. Sequence insert ends of large clones such as P1, YAC and BAC DNAs.
4. Isolate unknown 5' promoter control regions and 3' transcriptional terminators of cDNAs.
5. Isolate unknown 5' and 3' RACE from first-strand cDNAs.

Our patented technology relies on a combination of our degenerate random tagging primers (DRT) and customer-provided gene-specific primers (GSP). Typically, three nested gene specific primers (GSPa, GSPb, and GSPc) are required in the reaction for the amplification of flanking sequences.

 

DRT primers are universal binding primers consisting of 3 parts:

1. a degenerate sequence
2. a random sequence
3. a tagging sequence

All the DRT primers share the same tagging sequence, but have different random sequences. The success of the walking reaction relies on the successful binding of the DRT primers to the flanking sequence during the primary PCR. Our patented DRT primers, along with our optimized cycling conditions and PCR buffer, greatly enhance the amplification process.

We offer 2 different APAgene™ GOLD Genome Walking Kits. Both kits yield excellent results. BT901 is our newest kit and the one we recommend to all. BT501 is an older version that many of our loyal clients prefer.

 

BT901 (10 walk format)
This kit utilizes UAP primers which are hairpin-containing primers making non-specific amplification much less efficient compared to targeted sequences.

 

BT501 (10 walk format)
This kit utilizes 2 different buffers (Buffer I and Buffer II) which provide help in troubleshooting experiments. There is also a DRT Primer Digestion step.

APAgene™ GOLD Genome Walking Kits (require dry ice for shipping; recommended for Canada and USA)

Catalogue Number	# Walks	Price (USD)
BT501 or BT901	10	$189.00

Our new APAgene™ GOLD-RT Genome Walking Kits can be shipped at ROOM TEMPERATURE! The advantage is that shipping fees are reduced to a minimum compared to the significant dry ice shipping fees. For example, shipping to the UK would cost about $45 US.

Catalogue Number	# Walks	Price (USD)
BT901-RT	10	$229.00