DNA sequencing-Canada
(USA and International customers please
click here!)
To download chromatogram-viewing software:
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Turnaround time
The following turnaround time for single strand sequencing is based on a DNA
template sample of acceptable quality and on our current operational capacity: 1
day for samples requiring 1-2 reactions; average sequencing speed is 4 to 6 kb
per week.
Fees and conditions
1)
One run:
$28.00 per reaction, up to 1200 bases.
2)
Sequencing
large inserts:
6.0 cents per base for inserts requiring more than two reactions to cover insert
length (primer walking). Internal walking primer: $28 each (including design and
synthesis); $15 for the same primer that can be used for more than one clone.
3)
Editing fee:
No charge for single-strand sequencing. An editing fee of $7.50 per 500 bases
applies for double strand sequencing. All sequences are confirmed and edited
against the complementary strand, pair by pair.
4)
G-C rich
sample:
When the G-C content is higher than 65%, or the insert contains very strong
secondary structures that will affect the normal sequencing procedure,
additional fees (primers or/and reactions) will be added, upon customer
approval.
5)
Data
quality:
For the above price and with a normal insert, an accuracy of >99.00% (Li-Cor
standard) is guaranteed for single strand sequencing while a 100% match is
guaranteed for double strand sequencing. However, sequencing quality is directly
related to the sequence make-up of the insert and the quality of the sample.
Sample Requirements and Recommendations
Template
Preparation: Use of Qiagen kits, phenol-chloroform extraction or CsCl-density
gradient centrifugation is recommended. If total size (insert + vector) is >7
Kb, a maxi-prep is recommended. Purity: A 260/280 ratio and an agarose gel
picture of digested and undigested DNA samples should be provided. Shipping
conditions: Plasmid DNA can be sent in dry form, in water or in a buffer
solution. PCR products must be submitted in a dry state or in 70% ethanol to
prevent degradation.
Sample Quantity and Concentration
Requested quantity of template for a single reaction is approximately 0.3
picomoles. The corresponding amount is about 200ng/1Kb of total size (insert +
vector). Alternatively, the amount in ml can be calculated using the below
formula. Please note that although our read-length for a single reaction can
reach up to 1,200 bases under ideal conditions, the amount of template should be
calculated assuming a read-length of 700-900 bases. Also, please note that 2 to
3 times of the required amount might be required in case of repeated sequencing
reactions.
0.195 x Total size (Kb) / Sample concentration [ug/ul] = ul of sample needed
for 1 reaction
NOTE: It is recommended that the DNA concentration be estimated by running a gel
(more accurate than a spectrometer reading). Ideal concentration is >0.5 mg/ml.
If concentration is too low this will result in a blank signal or the signal
will be lost in the background. Clients bear responsibility for the descriptions
of their sample(s).
Pick-up service
Please contact us at 514.633.6006 for our courrier account
number 24 hours prior to pick-up. A local shipping fee of $10.00 will be
charged. This fee is waived for an order of $250.00 or more before taxes.
Please contact us for our account number with FedEx prior to pick-up.
Download order form