Fosmid Library-Random sheared

100,001 clones to 1 million clones - $4,995.00 US

Fosmid clones are similar in size (ca. 40kb) to cosmids, but, like BAC clones, they contain replicons derived from the F factor for DNA replication and segregation. Because of this property, they are present in low copy number and consequently more stable than cosmids. Fosmid libraries are commonly used for closing gaps in a whole genome sequence, physical maping, metagenomic and expression screening and more.

 

We offer:

The key step for fosmid library is preparation of inserts suitable for cloning into the fosmid vector. To achieve true randomness of inserts, our fosmid libraries are generated by random physical shearing of high molecular weight DNA (Please see Figure 1) using proprietary methods. A physically random sheared fosmid library will dramatically reduce the costs for downstream application(s) such as end-sequencing and screening.

Figure 1: Physically sheared HMW DNA (Lane 1: un-sheared, Lanes 2-4: sheared).




Figure 2: Size determination of fosmid clones.



We offer non-arrayed libraries and arrayed libraries (colony picking, replicate plates, high density filters). We also offer customer library screening for guaranteed positive clones. Copy control vector can be used for easy DNA purification from fosmid clones.