Full BAC clone sequencing

Our price: $1,499 / unit

One unit is equal to 10Kb (insert DNA + vector). The final cost will increase or decrease according to the final assembled genome size. Price does not include genome annotation.

Outline of the procedure:

1. Cell culture of BAC clones.
2. Extraction & Purification of BAC DNA.
3. Determination of BAC Size (vector plus insertion).
4. Construction of Random Shotgun Library.
5. Sequencing:
   1. Template DNA Extraction & Purification.
   2. Shotgun sequencing by universal primer.
   3. Real time annotation reports:
      1. Assemble
      2. Contig Alignment
      3. Bridge (Linking) Clone Identification
6. Cell culture of BAC clones.
   1. Determination of bridge clone sequence or, random amplified PCR products based on contig end specific primers.
   2. Average genome coverage > 99%.

Special offer:
Clients who have constructed and screened a BAC library with Bio S&T, or will do so at a future date, will benefit from a preferred rate.

For further details or a quote, please contact Areti Karadimos at areti@biost.coms