Superpools and Pools Package

DNA pools are a very useful tool that enable PCR screening at the client's facility. Here below is an example using a BAC library comprised of 75 x 384-well plates.

 

Deliverables

 

1.    75 Plate Pools (1plates/pool) (isolated DNA).

 

2.    5 Superpools ( isolated DNA; Superpool plates not included). Each of these Superpools will contain clones from the original plates as shown below:

 

1)       Superpool A = Original Plates 1-15

2)       Superpool B = Original Plates 16-30

3)       Superpool C = Original Plates 31-45

4)       Superpool D = Original Plates 46-60

5)       Superpool E = Original Plates 61-75

 

3.    80 Row pools from Superpool plates: 16 rows x 5 Superpool plates = 80 Superpool Row Pools (isolated DNA).

 

4.    120 Column pools from Superpool plates: 24 columns x 5 Superpool plates = 120 Superpool Column Pools (isolated DNA).

 

 

Highlights

 

-          DNA for all pools will be isolated from a 5ml culture (sufficient for 50-100 screenings).

-          Our pooling method is 3-dimensional.  Screening is straightforward and positive clones can be traced easily.

-          We will also store the cells for further DNA isolation should it be necessary.

 

 

Screening Method

 

Screen using the 5 Superpools first. For example, let’s assume Superpool A is positive. Continue by screening the 15 Plate pools corresponding to the positive Superpool (1 to 15 in this case).  Let’s assume that Plate pool 10 is positive. Continue by screening the 16 Row pools and 24 Column pools from Superpool A. If Row pool A is positive and Column pool 20 is positive then the positive clone is located in Plate 10- clone A20.

 

For further details or a quote, please contact Areti Karadimos at areti@biost.com