Partially purified tRNALys
Partially purified tRNALys
contains tRNALys1,2, tRNALys3 and other unknown tRNAs.
The partial purified tRNALys is extracted from human placenta with
the following procedures: (1) the extraction of total RNA by phenol-chloroform;
(2) the chromatogram extraction of total tRNA with DE52; and (3) the
chromatogram separation of total tRNALys from total tRNA with
DEAE-Sephadex (A50).
Aminoacylation of tRNA with H3 -lableled lysine using aminoacyl tRNA
synthetase from bovine liver is used for detection.
This product can be used to initiate reverse transcription or to carry out
hybridization in a specific, but an unquantitative manner.
For
long-term storage (years), it is recommended that this product be stored at -80
degrees at a concentration of no less than 0.5mg/ml. For short-term
storage, this product is stable at -20 degrees for a minimum of one year.
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