tRNALys1,2
tRNALys1,2 is a primer
of reverse transcriptase of human and simian spuma retroviruses, Visna virus,
CAEV, MPMV, squirrel monkey retrovirus and EIAV.
tRNALys1,2 is
purified from the human placenta using the following procedures: (1) the
extraction of total RNA by phenol-chloroform; (2) the chromatogram extraction
of total tRNA with DE52; (3) the chromatogram separation of total tRNALys
with DEAE-Sephadex (A50); (4) the chromatogram (HPLC/LC) separation
of tRNALys1,2 and tRNALys3 with RPC-5 column;
and (5) the chromatogram (HPLC/LC) purification of tRNALys1,2 with
C4-reverse column.
Detection
is performed by aminoacylation of tRNA with H3-lableled lysine using
aminoacyl tRNA synthetase from bovine liver.
For long-term storage (years), it is recommended that this product be stored at
-80 degrees at a concentration of no less than 0.5mg/ml. For short-term
storage, this product is stable at -20 degrees for a minimum of one year.
Please
contact us for more details!
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