tRNALys3
tRNALys3 is
a RT primer for HIV-1, HIV-2, SIV and FIV. tRNALys3 is purified from
human placenta with the following procedures: (1) total RNA extraction by
phenol-chloroform; (2) chromatogram extraction of total tRNA with DE52; (3)
chromatogram separation of total tRNALys with DEAE-Sephadex (A50);
(4) chromatogram (HPLC/LC) separation of tRNALys1,2and tRNALys3
with RPC-5 column; and (5) chromatogram (HPLC/LC) purification of tRNALys3
with C4-reverse column.
Detection is performed by
aminoacylation of tRNA with H3 -lableled lysine using aminoacyl tRNA
synthetase from bovine liver.
For long-term storage (years), it is recommended that this product be
stored at -80 degrees at a concentration of no less than 0.5mg/ml. For
short-term storage, this product is stable at -20 degrees for a minimum of one
year.
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