tRNALys3

tRNALys3 is a RT primer for HIV-1, HIV-2, SIV and FIV. tRNALys3 is purified from human placenta with the following procedures: (1) total RNA extraction by phenol-chloroform; (2) chromatogram extraction of total tRNA with DE52; (3) chromatogram separation of total tRNALys with DEAE-Sephadex (A50); (4) chromatogram (HPLC/LC) separation of tRNALys1,2and tRNALys3 with RPC-5 column; and (5) chromatogram (HPLC/LC) purification of tRNALys3 with C4-reverse column. 

Detection is performed by aminoacylation of tRNA with H3 -lableled lysine using aminoacyl tRNA synthetase from bovine liver.

For long-term storage (years), it is recommended that this product be stored at -80 degrees at a concentration of no less than 0.5mg/ml. For short-term storage, this product is stable at -20 degrees for a minimum of one year.

Please contact us for more details!