tRNATrp
tRNATrp is a primer of
reverse transcriptase of Avian retroviruses (except ASNV).
tRNATrp is purified from
human placenta with the following procedures: (1) the extraction of total RNA
by phenol-chloroform;(2) the chromatogram extraction of total tRNA with
DE52;(3) the chromatogram separation of total tRNATrp with
DEAE-Sephadex (A50); (4) the chromatogram (HPLC/LC) separation of tRNATrp
with RPC-5 column; (5) the chromatogram (HPLC/LC) purification of tRNATrp
with C4-reverse column.
Detection
is performed by aminoacylation of tRNA with H3-lableled tryptophan
using aminoacyl tRNA synthetase from bovine liver.
For long-term storage (years), it
is recommended that this product be stored at -80 degrees at a
concentration of no less than 0.5mg/ml. For short-term storage, this product is
stable at -20 degrees for a minimum of one year.
Please
contact us for more
details!
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