tRNATrp

tRNATrp is a primer of reverse transcriptase of Avian retroviruses (except ASNV).

tRNATrp is purified from human placenta with the following procedures: (1) the extraction of total RNA by phenol-chloroform;(2) the chromatogram extraction of total tRNA with DE52;(3) the chromatogram separation of total tRNATrp with DEAE-Sephadex (A50); (4) the chromatogram (HPLC/LC) separation of tRNATrp with RPC-5 column; (5) the chromatogram (HPLC/LC) purification of tRNATrp with C4-reverse column.

Detection is performed by aminoacylation of tRNA with H3-lableled tryptophan using aminoacyl tRNA synthetase from bovine liver.

For long-term storage (years), it is recommended that this product be stored at -80 degrees at a concentration of no less than 0.5mg/ml. For short-term storage, this product is stable at -20 degrees for a minimum of one year.

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