tRNAPro

tRNAPro is a primer of reverse transcriptase of ASNV, Leukemia and sarcoma virus (MuLV, MuSV, FSV, FLV, BLV, SSV, GaLV, HTLV-I and HTLV-II).

tRNAPro is purified from human placenta with the following procedures: (1) the extraction of total RNA by phenol-chloroform; (2) the chromatogram extraction of total tRNA with DE52; (3) the chromatogram separation of total tRNAPro with DEAE-Sephadex (A50); (4) the chromatogram (HPLC/LC) separation of tRNAPro with RPC-5 column; (5) the chromatogram (HPLC/LC) purification of tRNAPro with C4-reverse column.

Aminoacylation of tRNA with H3-lableled proline using aminoacyl tRNA synthetase from bovine liver is used for detection.


For long-term storage (years), it is recommended that this product be stored at -80 degrees at a concentration of no less than 0.5mg/ml. For short-term storage, this product is stable at -20 degrees for a minimum of one year.

 

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