Partially purified tRNAPro

tRNAPro is a primer of reverse transcriptase of ASNV, Leukemia and sarcoma virus (MuLV, MuSV, FSV, FLV, BLV, SSV, GaLV, HTLV-I and HTLV-II).

tRNAPro is extracted from human placenta with the following procedures: (1) the extraction of total RNA by phenol-chloroform; (2) the chromatogram extraction of total tRNA with DE52; and (3) the chromatogram separation of total tRNAPro from total tRNA with DEAE-Sephadex (A50).

Aminoacylation of tRNA with H3-lableled lysine using aminoacyl tRNA synthetase from bovine liver is used for detection.

This product can be used to initiate reverse transcription or to carry out hybridization in a specific, but an unquantitative manner. 

For long-term storage (years), it is recommended that this product be stored at -80 degrees at a concentration of no less than 0.5mg/ml. For short-term storage, this product is stable at -20 degrees for a minimum of one year.

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