Partially
purified tRNAPro
tRNAPro
is a primer of reverse transcriptase of ASNV,
Leukemia and sarcoma virus (MuLV, MuSV, FSV, FLV, BLV, SSV, GaLV, HTLV-I and
HTLV-II).
tRNAPro
is extracted from human placenta with the following procedures: (1) the
extraction of total RNA by phenol-chloroform; (2) the chromatogram extraction
of total tRNA with DE52; and (3) the chromatogram separation of total
tRNAPro from
total tRNA with DEAE-Sephadex (A50).
Aminoacylation of tRNA with H3-lableled lysine using aminoacyl tRNA
synthetase from bovine liver is used for detection.
This product can be used to initiate reverse transcription or to carry out
hybridization in a specific, but an unquantitative manner.
For
long-term storage (years), it is recommended that this product be stored at -80
degrees at a concentration of no less than 0.5mg/ml. For short-term
storage, this product is stable at -20 degrees for a minimum of one year.
Please
contact us for more details!
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