Aminoacyl-tRNA Synthetase

Aminoacyl-tRNA Synthetase is suspended in 10 mM Tris buffer (pH 7.6) containing 50% glycerol, 10 mM MgCl2, 10 mM KCl and 30 mM 2-mercaptoethanol.

    • Activity: >20 U/ul.

    • Quantity: 1,000 U (sufficient for 200 reactions; 5 U needed/reaction)

    • Source: Bovine liver.

    • Storage: -20 degrees.

    • Protocol: Aminoacyl-tRNA Synthetase

Application:

This enzyme is used to catalyze the aminoacetylation of tRNAs with its correspondent amino acid. Testing indicates that this enzyme can catalyze the aminoacetylation of lysine, glutamic acid, proline and tryptophane with their correspondent tRNA. Aminoacetylation of other amino acid-tRNAs should be tested.

Unit definition:

One unit will activate and attach 0.1 picomole of H3-labeled lysine amino acid to tRNA in 30 minutes at 37 degress. The activity of this enzyme is about 15 times higher then the one offered by our competitors: 5 units of this enzyme can suffice for a 60ml reaction volume.

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