Aminoacyl-tRNA Synthetase
Aminoacyl-tRNA Synthetase
is suspended in 10 mM Tris buffer (pH 7.6) containing 50% glycerol, 10 mM
MgCl2, 10 mM KCl and 30 mM 2-mercaptoethanol.
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Activity: >20
U/ul.
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Quantity:
1,000 U (sufficient for 200 reactions; 5 U needed/reaction)
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Source:
Bovine liver.
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Storage:
-20 degrees.
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Application:
This
enzyme is used to catalyze the aminoacetylation of tRNAs with its correspondent
amino acid. Testing indicates that this enzyme can catalyze the
aminoacetylation of lysine, glutamic acid, proline and tryptophane with their
correspondent tRNA. Aminoacetylation of other amino acid-tRNAs should be
tested.
Unit definition:
One unit will activate and attach 0.1 picomole of H3-labeled lysine
amino acid to tRNA in 30 minutes at 37 degress. The activity of this enzyme is
about 15 times higher then the one offered by our competitors: 5 units of this
enzyme can suffice for a 60ml reaction volume.
Please
contact us for more details!
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