- 基因服務 - pESAC13??

pESAC13??

Bio S?T???????pESAC13?????????pESAC13 PAC????????????????NAICONS??????????????????Streptomyces sp.??????Actinomycete sp.????????DNA?????????????????????????????????????

ESAC (E. coli-Streptomyces Artificial Chromosome) ??????????????????????????????????????????????????????????????

pESAC13?pPAC-S1??????????????ESAC?? ?Sosio et al., 2000?????????RK2????Simon et al., 1983??oriT?760bp DNA?????pPAC-S1?M. Sosio et al., unpublished??BstXI??????????

 

Latest articles:

BAC cloning and heterologous expression of a giant biosynthetic gene cluster encoding antifungal neotetrafibricin in streptomyces rubrisoli.

Biosynthesis of Aurodox, a Type III Secretion System Inhibitor from Streptomyces goldiniensis

Discovery, isolation, heterologous expression and mode-of-action studies of the antibiotic polyketide tatiomicin from Amycolatopsis sp. DEM30355

Streptomyces BAC Cloning of a Large-Sized Biosynthetic Gene Cluster of NPP B1, a Potential SARS-CoV-2 RdRp Inhibitor

 

pESAC13???????????????????????????????????????????????????????????????????????????????

 

? 1: pESAC????

pESAC vector map

????

  1. ????????
  2. ??????????????
  3. ??????????????HMW ??DNA?
  4. ??????????????????????
  5. ????????GC??????????????
  6. ???BAC????????????????
  7. ??????????????????
  8. ??????????????
  9. PAC???????????????????????
  10. ????????????

 

? 1: ???????PAC??DNA?????

Starting material Average Insert size
Streptomyces sp. 130 Kb
Streptomyces sp. 145 Kb
Streptomyces sp. 125 Kb
Actinomycete sp. 155 Kb
Streptomyces sp. 139 Kb

 

? 2?DraI???PFG???????????????155Kb?PAC???????

Insect clones