- 基因服務 - 逆转录病毒研究

逆转录病毒研究

Bio S&T is the only commercial provider of highly purified human tRNAs.

RNA is most widely used as the primer of retroviral reverse transcriptases. The 3’ end of the tRNA binds to the complementary binding site on the viral RNA therefore initiating reverse transcription.

  1. tRNALys3 is the primer of HIV-I, HIV-II, SIV and FIV.
  2. tRNALys1,2 are the primers of BLV, HTLV-I and II, SSV, FLV, VV, CAEV, MPMV, SMR and EIAV.
  3. tRNATrp and tRNAPro are the primers of ASNV, SSV, FLV, BLV, MLV, HTLV-I and II.

Since it has been well documented that artificial tRNA does not posses the same biological properties as natural tRNA, human purified tRNAs are largely in demand for retroviral research, especially HIV related research.

The extraction and purification of tRNA from a natural source such as human placenta is a highly complex and delicate procedure.

Here are our unique products:

 

Product description Catalogue No. Unit size
tRNALys3, pure Source: Human placenta Purity: HPLC grade BH101 1 ug
tRNALys1,2, pure Source: Human placenta Purity: HPLC grade BH102 1 ug
tRNALys, partial Source: Human placenta Purity: DEAE grade BH103 10 ug
tRNAPro, pure Source: Human placenta Purity: HPLC grade BH201 1 ug
tRNAPro, partial Source: Human placenta Purity: DEAE grade BH202 10 ug
tRNATrp, pure Source: Human placenta Purity: HPLC grade BH301 1 ug
tRNATrp, partial Source: Human placenta Purity: HPLC grade BH302 10 ug
Total tRNA Source: Human placenta BH401 100 ug
Aminoacyl-tRNA Synthetase (from bovine liver) BH501 1,000 ug