Bio S?T???????pESAC13?????????pESAC13 PAC????????????????NAICONS??????????????????Streptomyces sp.??????Actinomycete sp.????????DNA?????????????????????????????????????
ESAC (E. coli-Streptomyces Artificial Chromosome) ??????????????????????????????????????????????????????????????
pESAC13?pPAC-S1??????????????ESAC?? ?Sosio et al., 2000?????????RK2????Simon et al., 1983??oriT?760bp DNA?????pPAC-S1?M. Sosio et al., unpublished??BstXI??????????
Latest articles:
Biosynthesis of Aurodox, a Type III Secretion System Inhibitor from Streptomyces goldiniensis
pESAC13???????????????????????????????????????????????????????????????????????????????
? 1: pESAC????
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- ????????
- ??????????????
- ??????????????HMW ??DNA?
- ??????????????????????
- ????????GC??????????????
- ???BAC????????????????
- ??????????????????
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- PAC???????????????????????
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? 1: ???????PAC??DNA?????
Starting material | Average Insert size |
Streptomyces sp. | 130 Kb |
Streptomyces sp. | 145 Kb |
Streptomyces sp. | 125 Kb |
Actinomycete sp. | 155 Kb |
Streptomyces sp. | 139 Kb |
? 2?DraI???PFG???????????????155Kb?PAC???????