pESAC13??

Bio S?T???????pESAC13?????????pESAC13 PAC????????????????NAICONS??????????????????Streptomyces sp.??????Actinomycete sp.????????DNA?????????????????????????????????????

ESAC (E. coli-Streptomyces Artificial Chromosome) ??????????????????????????????????????????????????????????????

pESAC13?pPAC-S1??????????????ESAC?? ?Sosio et al., 2000?????????RK2????Simon et al., 1983??oriT?760bp DNA?????pPAC-S1?M. Sosio et al., unpublished??BstXI??????????

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pESAC13???????????????????????????????????????????????????????????????????????????????

? 1: pESAC????

????

1. ????????
2. ??????????????
3. ??????????????HMW ??DNA?
4. ??????????????????????
5. ????????GC??????????????
6. ???BAC????????????????
7. ??????????????????
8. ??????????????
9. PAC???????????????????????
10. ????????????

? 1: ???????PAC??DNA?????

? 2?DraI???PFG???????????????155Kb?PAC???????