RapidRun™ 200-3000 bp DNA Ladder is designed for agarose gel electrophoresis enabling quick size estimation and approximate quantification of double-stranded DNA. The ladder is comprised of 8 individual DNA fragments derived from proprietary plasmid vectors to ensure the bands’ stability, accuracy and clarity: 3000, 2000, 1500, 1000, 800, 600, 400 and 200bp. The 1 Kb band is more intense and serves as a reference band.
Description
| Cat. number | # M200-8R | # M200-8RL |
| Number of loads | 100 applications | 500 applications |
| Quantity | 50 µg | 5 X 50µg |
| Volume | 600 µL | 5 X 600 µL |
| Concentration | 500ng/6µl | 500ng/6µl |
Recommendations for use
6 µL per loading is recommended. The ladder is ready to use format with storage buffer and loading dye (10 mM Tris-HCl (pH 7.6), 0.06% Cresol Red, 0.12% Orange G, 60% glycerol and 60 mM EDTA).
Quality control
Agarose gel electrophoresis analysis shows well-defined bright and sharp bands between 200-3000bp. The DNA concentration is determined by spectrophotometry. Incubation of 6ul of the RapidRun™ Ladder in restriction enzyme buffer overnight at 37°C shows absence of visible degradation on an ethidium bromide-stained agarose gel.
Storage
Product is shipped at ambient temperature. Store at -20°C. Product is stable for 1 year at -20°C or 6 months at ambient temperature.