pESAC-13 libraries

Bio S&T is the exclusive worldwide provider of pESAC13 PAC libraries. This unique vector (licensed exclusively to Bio S&T by NAICONS) is used to create Streptomyces sp. and Actinomycete sp. libraries. These libraries can then be screened for clones that will be positive for the desired gene clusters of biosynthetic pathways.

Latest articles:

Multiplexed mobilization and expression of biosynthetic gene clusters

BAC cloning and heterologous expression of a giant biosynthetic gene cluster encoding antifungal neotetrafibricin in streptomyces rubrisoli.

Biosynthesis of Aurodox, a Type III Secretion System Inhibitor from Streptomyces goldiniensis

Discovery, isolation, heterologous expression and mode-of-action studies of the antibiotic polyketide tatiomicin from Amycolatopsis sp. DEM30355

Streptomyces BAC Cloning of a Large-Sized Biosynthetic Gene Cluster of NPP B1, a Potential SARS-CoV-2 RdRp Inhibitor

Highlights

1. HMW DNA isolation is included.
2. pESAC-13 vector (BamHI) vector and DH10B cells will be used for construction.
3. We guarantee a minimum 20X BAC library which provides a >99.99% probability of finding the target sequence. Amount of clones received is based on a guaranteed coverage and 120Kb average insert size.
4. Clones will be arrayed and amplified in 5 x 384-well plates (1 unique clone/well).
5. QC by HMW DNA enzymatic digestion are included. Quality control (average insert size and percentage of empty clones) will be evaluated using randomly selected clones.
6. Customized screening for positive clones can be done at Bio S&T.

Our strengths

• We guarantee coverage.
• High average insert and even insert size distribution.
• We perform HMW DNA extraction at our facilities.
• We have experience with plant, fungal, bacterial and animal libraries.
• We have extensive experience with GC-rich organisms and marine bacteria.
• Our arrayed BAC libraries are shipped in bar-coded microplates.
• We have extensive experience shipping worldwide.
• We provide technical support.
• PAC libraries are the client's property and are never used internally, sold or distributed.
• We provide references

Table 1: A few PAC libraries constructed in the past with obtained average insert sizes.

Figure 2: PFG following DraI digestion of PAC clones (Actinomycete sp.) with an average insert size of 155Kb.